The CRISALIS, funded by the RCMI Program in Health Disparities Research at Meharry Medical College, physically and operationally co-locates the equipment and personnel of our shared research resources, providing synergistic services to enhance research productivity and outcomes. Intentionally evoking a metamorphic process, CRISALIS is ready to support transdisciplinary research spanning micro to macro scales of inquiry in pursuit of health equity. Services, pricing, up to date equipment lists, and contact information are available at the iLabs link below under the Facility/Resource URL tab – account required.
The Biostatistics Core has its academic home in the School of Graduate Studies and provides statistical support and services (i.e. statistical methodology and study design, sample size determination/power analysis, statistical analysis plan, data analytics, interpretation of results) using state of the art statistical methods and software to all Meharry faculty, staff, and students engaged in research as well as collaborators outside Meharry Medical College.
Consulting services are offered for a fee. The Biostatistics Core will determine the time it will take to complete the project, and present the associated fee for the requested support to the investigator. Collaborative projects should fund a biostatistician’s effort in one of the following ways:
The Meharry RCMI BioMedical Informatics Core provides services and expertise in existing & emerging research technologies for Bioinformatics, Computational Biology, Clinical & Health Informatics, Band Multi-Omics data analysis.
We provide analysis and training for:
Proteomics analysis is a powerful approach in discovering and understanding an organism’s functions and responses. Typically, it involves: 1) identify endogenous proteins for a given specie, 2) provide relative quantification of these proteins, and 3) characterize post-translational modifications (e.g. phosphorylation, glycosylation, sumoylation, etc.). Proteomics sub-core specializes in bottom-up LC-MS based global proteomics analysis. In addition, we offer targeted small molecule LC-MS analysis and a variety of protein analysis techniques (FPLC, UHPLC, and SPR).
For inquiries, contact Proteomics Core Director, Victor Paromov PhD, email@example.com
Physically separate from the rest of CRISALIS, our biosafety-level 3 lab is still operationally integrated with CRISALIS workflows. The BSL-3 provides a safe and appropriate environment for the investigation of infectious agents such as HIV. The design of the lab limits exposure to such agents and laboratory personnel have specific training in proper handling procedures.
There are four biosafety cabinets and six CO2 incubators inside the lab, accommodating up to six researchers simultaneously. The free-standing Beckman Optima L-90K Ultracentrifuge has an SW60Ti and an SW41 rotor for sample processing.
Four flow cytometers are available by reservation.
The CytoFLEX S Flow Cytometer from Beckman Coulter equipped with the advanced new avalanche photodiode (APD) detecting system and Violet Side Scatter (VSSC) option, which provides the CytoFLEX with exceptional detection capabilities, large dynamic range to resolve dim and bright populations in the same sample, and power of nanoparticle detection. It comes with a 96-well plate loader for automated sampling, four lasers (405nm, 488 nm, 561nm, and 638nm), enabling up to 13 parameters simultaneously. The CytoFLEX S is located in a Biosafety Cabin in the BSL3 lab on the fifth floor of the Hubbard Hospital Building. It can be used to analyze samples infected by pathogens (equal or below BSL3 level).
The Luminex Amnis® CellStream Flow Cytometry System, featuring patented Amnis® Time Delay Integration (TDI) sensory technology, is a unique flow cytometry system that provides unparalleled sensitivity and flexibility. Our CellStream comes standard with a 96-well plate loader for automated sampling, four lasers (405nm, 488 nm, 532nm, and 642nm), enabling up to 17 parameters. The CellStream differs from all other flow cytometers in that the signal is detected by a charge-coupled device (CCD)-camera using TDI sensory technology unique to the Amnis product portfolio. TDI enables high fluorescence sensitivity to provide excellent population resolution and additional morphological parameters not possible with a conventional cytometer. In addition, the high numerical aperture (NA) lens yields exceptional forward and side scatter sensitivity for the detection of small particles. The CellStream System can be used for traditional flow cytometry applications as well as cutting-edge, high parameter data generation and analysis. The CellStream is located in Room 4003 of the West Basic Science Building.
The FACSAria III from Becton Dickinson includes three solid-state lasers: blue (488nm), red (633nm), and violet (405). The system has the ability to detect five colors from the blue laser, two colors from the red laser, and two colors from the violet laser for a total of nine simultaneous fluorescent colors. The detection optics includes BD’s patented fiber-coupled octagon and trigon technology. The digital acquisition system allows inter-and intra-beam compensation as well as the ability to threshold on any parameter. The automated cell deposition unit (ACDU) can accommodate slides and 6, 24, 48, 96 and 384-well plates.
The FACSCalibur from Becton Dickinson, a four-color, dual-laser, benchtop flow cytometry system, provides cell analysis. This fully integrated multiparameter system can also perform a range of functions. To meet investigators’ throughput and productivity requirements, the device comes with innovative dual laser technology, powerful software, and an automated sample loader option.
Time on these machines can be reserved through our online reservation forms: Aria, CytoFlex or Amnis. New reservations can be modified within 30 minutes of creation. Changes after 30 minutes need to be made by CRISALIS staff. Please contact the Flow Cytometry administrator to do so.
Cell analysis is $60/hr, 15 minute minimum.
Cell sorting is $90/hr + $45 setup fee.
The Gene Editing Core at Meharry Medical College provides internal and external investigators access to state-of-the-art genome editing technologies by assisting with the generation of genetically knockout or knockin cells in a time- and cost-effective manner. We have years of experience with knockout and knockin genes in cell lines and progenitor cells with high efficiencies. Working with investigators, we assist in designing guide RNAs, preparing reagents, transfection/infection, cloning, screening and off-target evaluation. For a free consultation, contact Dr. Bindong Liu at firstname.lastname@example.org
The Fluorescence Microscopy and Morphology Core provides expertise and state-of-the-art instrumentation for performing fluorescence and bright-field microscopy to investigators at Meharry. The core equipment includes confocal, wide-field, TIRF, and super resolution microscopes. Stage-top incubators for imaging at 370C and 5% CO2 are available for live-cell imaging. The core also has multimode microplate readers and a variety of fluorescent and bright-field imagers with options of whole slide imaging, long term time-lapse experiments, and more. The core has resources and expertise for immunofluorescence staining of cells and fixed tissues. Core staff offers consultation on instrumentation and experimental design, instrument training, and collaboration opportunities.
• A1R laser and resonance scanning confocal microscope (Nikon)
• TE2000E wide-field fluorescence microscope (Nikon)
• Ti2 wide-field fluorescence microscope (Nikon)
• Super-resolution STORM system (Nikon)
• TIRF microscope (Nikon)
• NIS Elements Advanced Research imaging and analysis software (Nikon)
• Cytation 5 cell imaging multimode reader (BioTek)
• Neo2 hybrid multimode microplate reader (BioTek)
• EVOS fluorescence microscope (Thermo Fisher)
• BZ-X700 fluorescence microscope (Keyence)
• Assisted Imaging: Scheduled in advance through iLabs, staff will assist you with your experiment during regular weekday hours.
• Unassisted Imaging: Users with completed equipment training may book microscope sessions through iLabs. Sessions are available 24/7.
Human Tissue Acquisition and Pathology services coordinates with Meharry clinics and Nashville General Hospital to offer clinical specimen, histology, and immunohistochemistry support for clinical and translational researchers. Only remnant tissue samples not needed for diagnostic purposes are collected. Normal, neoplastic, and other diseased tissues are available fresh or snap frozen or in a variety of fixatives.
Human and animal pathology diagnostic services and microscopic interpretation of Hematoxylin and Eosin, special stain, and immunohistochemistry are also available, as well as digital photography and descriptions for manuscripts. The existing specimen catalogue and service pricing are available from Michael Izban, email@example.com.
The Endocrine Core Laboratory performs a comprehensive list of routine and specialized endocrine tests on plasma/serum samples and conditioned media for government funded investigators. An endocrinologist is available for consultation and interpretation of test results.
Immulite 1000 from Siemens
Our main instrument for hormone assays, the Immulite 1000 is automated for tests of moderated complexity with an expandable test menu.
· Uses chemiluminescence reagents
· Has programmed standard curve set in each kit
· Reagents/assay kit are specific to each kit lot
· Kit adjustments needed every two weeks
Perkin Elmer Gamma Counter (The Wizard)
Our auxiliary instrument, is the latest and most advanced gamma counter. It can operate as an automatic stand-alone CPM, or it can be used to do extensive data evaluation with its own internal RiaCalc WIZ program or be linked up to an external PC and use the power of a MultiCalc program.
The Endocrine Core Laboratory provides the following assays for service at the prices shown.
|Test Name||Fee/Sample ($)|
|Follicle Stimulating Hormone (FSH)||20.00|
|17 a-OH Progesterone||33.00|
|Sex Hormone Binding Globulin (SHBG)||28.00|
|Thyroid Stimulating Hormone (TSH)||30.00|
|Thyroid Hormone (T3/T4)||30.00|
For more information about assays, sample collection, sample shipment, prices and assay schedule, please contact the Endocrine Core Laboratory by phone, 615.327.5902, 615.327.5714 or fax, 615.327.6296.
Discounted prices for testing can be negotiated for large studies.
Molecular Biology Core provides convenient access to molecular biological shared equipment, as well as training and technical assistance for use of the instruments. Equipment and resources for molecular biology applications are readily available and reside in the Core Laboratory or in common equipment rooms and corridors in the West Basic Science building. Training in the proper use of equipment is provided by MRRCC staff and also as needed by vendor technical personnel.
• Synergy Neo2 (BioTek)
• xMark Microplate Spectrophotometer (Bio-Rad)
• Synergy HT (BioTek)
• Odyssey Model 1785 Infrared Imaging System
• ChemiDoc Gel Imaging Systems (BioRad)
• CFX96 Touch Real-Time PCR Systems (BioRad)
• CFX384 Touch Real-Time PCR System (BioRad)
• QIAxpert (Qiagen)
• QiaCube (Qiagen)
• NGC Quest 10 Chromatography System (BioRad)
• HPLC Chromatography System (Agilent)
• NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher)
• NanoDrop 2000c Spectrophotometer (Thermo Fisher)
• 4150 TapeStation Bioanalyser (Agilent)
• WES Automated Western Blot (Protein Simple)
• 405 TS Microplate washer (BioTek)
• Trans-Blot Turbo Transfer Systems (Bio-Rad)
• TC20 and TC10 Automated Cell Counters (Bio-Rad)
• ZetaView Nanoparticle Analyzer (ParticleMetrix)
• OpenSPR 4 (Nicoya)
Common facilities and resources:
• Sorvall Discovery 150 Micro-Ultracentrifuge (Thermo Fisher)
• Optima XPN-100 Ultracentrifuges (Beckman Coulter)
• Sorvall Lynx 4000 Superspeed Centrifuge (Thermo Fisher)
• Biological safety cabinet – class II (LABCONCO)
• CO2 incubators
• Savant SPD111V Speedvac Concentrator
• Avanti J-15, High Speed Benchtop Centrifuge (Beckman Coulter)
• Various benchtop microcentrifuges for tubes, plates, and tube strips.
• Analytical scale (Fisher)
• Milli-Q Advantage Water Purification System (Millipore)
• Fridges and freezers
There are two analysis computers available for researchers use and to analyze data from the Core equipment. Specialized software available:
• NIS elements (Nikon)
• Gen5 analysis software with imaging module (BioTek)
• BZ-X analyzer (Keyence)
• and more.
School of Medicine
Center for AIDS Health Disparities Research
School of Medicine
Office for Research and Innovation
School of Medicine
Center for Molecular & Behavioral Neuroscience
School of Medicine
Health Disparities Research Center of Excellence
Acting Scientific/Managing Director, Translational Pathology Shared Resource/Tissue Acquisition Core
School of Medicine
School of Graduate Studies and Research
National Center for Medical Education Development & Research
Staff Scientist, Flow Cytometry
Office for Research and Innovation
Optogenetic-induced multimerization of the dopamine transporter increases uptake and trafficking to the plasma membrane.
Shalonda M Ingram, Tanu Rana, Ashley M Manson, Faisal M Yayah, Evan G B Jackson, Christopher Anderson, Benem-Orom Davids, J Shawn Goodwin
Mechanistic role of antioxidants in rescuing delayed gastric emptying in high fat diet induced diabetic female mice.
Chethan Sampath, Derek Wilus, Mohammad Tabatabai, Michael L Freeman, Pandu R Gangula
An introduction to new robust linear and monotonic correlation coefficients.
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KDM5B Is Essential for the Hyperactivation of PI3K/AKT Signaling in Prostate Tumorigenesis.
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The Effects of Perceived Stress and Cortisol Concentration on Antiretroviral Adherence When Mediated by Psychological Flexibility Among Southern Black Men Living with HIV.
Robert L Cooper, Lauren L Brown, Mohammad Tabatabai, David W Haas, Bryan E Shepherd, Hector F Myers, Ryan D Edgerton, Castro Bonny, Julia A Watson, Vladimir Berthaud
Modeling dynamics of fatal opioid overdose by state and across time.
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