The CRISALIS, funded by the RCMI Program in Health Disparities Research at Meharry Medical College, physically and operationally co-locates the equipment and personnel of our shared research resources, providing synergistic services to enhance research productivity and outcomes. Intentionally evoking a metamorphic process, CRISALIS is ready to support transdisciplinary research spanning micro to macro scales of inquiry in pursuit of health equity.
The Biostatistics Core has its academic home in the School of Graduate Studies and Research and provides statistical services to all Meharry faculty, staff and students engaged in research as well as collaborators outside Meharry.
Consulting services are offered for a fee. The Biostatistics Core will determine the time it will take to complete the project, and present the associated fee for the requested support to the investigator. Collaborative projects should fund a biostatistician’s effort in one of the following ways:
For inquiries, contact Mr. Derek Wilus, email@example.com
CRISALIS Bioinformatics, BioMedical Informatics, and Proteomics services incorporate existing & emerging research technologies for Bioinformatics, Computational Biology, Clinical & Health Informatics, Big Data and “Omics” level data analysis.
We provide analysis and training for:
Physically separate from the rest of CRISALIS, our biosafety-level 3 lab is still operationally integrated with CRISALIS workflows. The BSL-3 provides a safe and appropriate environment for the investigation of infectious agents such as HIV. The design of the lab limits exposure to such agents and laboratory personnel have specific training in proper handling procedures.
There are four biosafety cabinets and six CO2 incubators inside the lab, accommodating up to six researchers simultaneously. The free-standing Beckman Optima L-90K Ultracentrifuge has an SW60Ti and an SW41 rotor for sample processing.
Four flow cytometers are available by reservation.
The CytoFLEX S Flow Cytometer from Beckman Coulter equipped with the advanced new avalanche photodiode (APD) detecting system and Violet Side Scatter (VSSC) option, which provides the CytoFLEX with exceptional detection capabilities, large dynamic range to resolve dim and bright populations in the same sample, and power of nanoparticle detection. It comes with a 96-well plate loader for automated sampling, four lasers (405nm, 488 nm, 561nm, and 638nm), enabling up to 13 parameters simultaneously. The CytoFLEX S is located in a Biosafety Cabin in the BSL3 lab on the fifth floor of the Hubbard Hospital Building. It can be used to analyze samples infected by pathogens (equal or below BSL3 level).
The Luminex Aminis® CellStream Flow Cytometry System, featuring patented Amnis® Time Delay Integration (TDI) sensory technology, is a unique flow cytometry system that provides unparalleled sensitivity and flexibility. Our CellStream comes standard with a 96-well plate loader for automated sampling, four lasers (405nm, 488 nm, 532nm, and 642nm), enabling up to 17 parameters. The CellStream differs from all other flow cytometers in that the signal is detected by a charge-coupled device (CCD)-camera using TDI sensory technology unique to the Amnis product portfolio. TDI enables high fluorescence sensitivity to provide excellent population resolution and additional morphological parameters not possible with a conventional cytometer. In addition, the high numerical aperture (NA) lens yields exceptional forward and side scatter sensitivity for the detection of small particles. The CellStream System can be used for traditional flow cytometry applications as well as cutting-edge, high parameter data generation and analysis. The CellStream is located in Room 4003 of the West Basic Science Building.
The FACSAria III from Becton Dickinson includes three solid-state lasers: blue (488nm), red (633nm), and violet (405). The system has the ability to detect five colors from the blue laser, two colors from the red laser, and two colors from the violet laser for a total of nine simultaneous fluorescent colors. The detection optics includes BD’s patented fiber-coupled octagon and trigon technology. The digital acquisition system allows inter-and intra-beam compensation as well as the ability to threshold on any parameter. The automated cell deposition unit (ACDU) can accommodate slides and 6, 24, 48, 96 and 384-well plates.
The FACSCalibur from Becton Dickinson, a four-color, dual-laser, benchtop flow cytometry system, provides cell analysis. This fully integrated multiparameter system can also perform a range of functions. To meet investigators’ throughput and productivity requirements, the device comes with innovative dual laser technology, powerful software, and an automated sample loader option.
Time on these machines can be reserved through our online reservation form. New reservations can be modified within 30 minutes of creation. Changes after 30 minutes need to be made by CRISALIS staff. Please contact the Flow Cytometry administrator to do so.
Cell analysis is $60/hr, 15 minute minimum.
Cell sorting is $90/hr + $45 setup fee.
Light microscopy services provide expertise and state-of-the-art instrumentation for fluorescence and bright-field microscopy and immunocytochemistry. Our staff offers consultation on instrumentation and experimental design, instrument training, and collaboration opportunities. We stay on the cutting edge of technology, skills, and knowledge in the field, and work hard at a highly collaborative environment. We train users to be independent and work with researchers until competency on the equipment is achieved.
Human Tissue Acquisition and Pathology services coordinates with Meharry clinics and Nashville General Hospital to offer clinical specimen, histology, and immunohistochemistry support for clinical and translational researchers. Only remnant tissue samples not needed for diagnostic purposes are collected. Normal, neoplastic, and other diseased tissues are available fresh or snap frozen or in a variety of fixatives.
Human and animal pathology diagnostic services and microscopic interpretation of Hematoxylin and Eosin, special stain, and immunohistochemistry are also available, as well as digital photography and descriptions for manuscripts. The existing specimen catalogue and service pricing are available from Michael Izban, firstname.lastname@example.org.
The Endocrine Core Laboratory performs a comprehensive list of routine and specialized endocrine tests on plasma/serum samples and conditioned media for government funded investigators. An endocrinologist is available for consultation and interpretation of test results.
Immulite 1000 from Sienmens
Our main instrument for hormone assays, the Immulite 1000 is automated for tests of moderated complexity with an expandable test menu.
· Uses chemiluminescence reagents
· Has programmed standard curve set in each kit
· Reagents/assay kit are specific to each kit lot
· Kit adjustments needed every two weeks
Perkin Elmer Gamma Counter (The Wizard)
Our auxiliary instrument, is the latest and most advanced gamma counter. It can operate as an automatic stand-alone CPM, or it can be used to do extensive data evaluation with its own internal RiaCalc WIZ program or be linked up to an external PC and use the power of a MultiCalc program.
The Endocrine Core Laboratory provides the following assays for service at the prices shown.
|Test Name||Fee/Sample ($)|
|Follicle Stimulating Hormone (FSH)||20.00|
|17 a-OH Progesterone||33.00|
|Sex Hormone Binding Globulin (SHBG)||28.00|
|Thyroid Stimulating Hormone (TSH)||30.00|
|Thyroid Hormone (T3/T4)||30.00|
For more information about assays, sample collection, sample shipment, prices and assay schedule, please contact the Endocrine Core Laboratory by phone, 615.327.5902, 615.327.5714 or fax, 615.327.6296.
Discounted prices for testing can be negotiated for large studies.
Primarily for DNA sequencing, Molecular Biology services include training and technical assistance for use of shared equipment and provides convenient access to molecular biological reagents. Training in the proper use of equipment is provided by the Laboratory Coordinator and also as needed by vendor technical personnel. The core maintains in-house freezers and supply cabinets stocked with many commonly used molecular biological reagents from Invitrogen.
The DNA Sequencing service provides accurate, long read, and fast turnaround DNA sequencing service. Samples are processed using BigDye terminator chemistry, and sequences are generated by an ABI 3100 Avant Genetic Analyzer, a high through-put capillary sequencer that generates high quality DNA sequence data with consistently long reads, typically 600-800 bases. The ABI 3100 is a capillary electrophoresis DNA sequence system with four capillaries. The instrument is fully automated from sample loading to data analysis.
Sequence data obtained are submitted electronically back to users for analysis. Most plasmids have sites for universal primers, such as M13 (forward and reverse), T7, T3, and SP6, which the CRISALIS keeps on-hand. Investigators using custom primers must submit primers with the template DNA. Purified PCR products are sequenced directly without sub-cloning. Usually, investigators submit custom primers with the PCR templates. To reduce the cost for researchers that need to sequence hundreds of samples, for example in screening projects, the Core offers a Load-Only option. The researcher is responsible for generating the sequencing reaction and the facility provides the sequence results back to the researcher.
School of Medicine
Center for AIDS Health Disparities Research
School of Medicine
Office for Research and Innovation
School of Medicine
Center for Molecular & Behavioral Neuroscience
Acting Scientific/Managing Director, Translational Pathology Shared Resource/Tissue Acquisition Core
School of Medicine
School of Graduate Studies and Research
National Center for Medical Education Development & Research
Staff Scientist, Flow Cytometry
Office for Research and Innovation
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